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17th Annual International 12-Day Short Course on 3D Microscopy of Living Cells - June 9 - 21, 2012 - Vancouver, BC, Canada
  Date: June 9-21, 2012
Location: University of British Columbia, Vancouver BC, Canada
Organized by:
Prof. James Pawley, University of Wisconsin-Madison
in association with the UBC Brain Research Centre and the Departments of Pharmacology and Physiology
University of British Columbia, Vancouver, BC, Canada
THE PURPOSE OF THE COURSE
Modern methods of 3D light microscopy promise a revolutionary improvement in our ability to view living cells. To help convert this promise to reality for a wider selection of biological scientists, the organizers have designed an intensive twelve-day residential course concentrating on all aspects of the 3D Microscopy of Living Cells. Sponsored by the Brain
Research Centre and the Departments of Pharmacology and Physiology at the University of British Columbia, it will be held June 9 - 22, 2012. The course includes 4 days on 2D techniques, 5 days of 3D techniques and 3 days on 3D data measurement and display. It includes everything from basic microscopy to confocal, multiphoton and STED microscopy. A half-day Pre-course is offered for those wishing to brush up on (very!) basic optics.
Topics at the Living-Cell Course include:
Quantitative 2D light microscopy
3D Imaging in confocal and widefield
Fluorescent and backscattered-light signals
Digitization: The Nyquist Criterion
Poisson noise, QE and S/N.
Lasers and laser tweezers
Objectives and automatic aberration correction
Scanning-systems: AODs, mirrors, disks
Deconvolution of widefield and confocal data
Detectors: operation and performance
Optimal pinhole size/photon efficiency
Dye design, characteristics and use
How to keep your cells alive
Multi-photon excitation
Calcium and pH imaging
PALM, FRET, FLIM, STED and TIRF
Display and measurement of 3D data
For more information and application please visit www.3dcourse.ubc.ca/2012
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