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2nd South-East Asian Course on "F-techniques: FCS, FCCS, FLCS, FRET, FLIM and FRAP" - July 16-20, 2012 - Singapore
  Course organisers:
Institute of Medical Biology Microscopy Unit (IMU),
Biopolis, Singapore
PicoQuant GmbH, Germany
Aim and purpose
The course is intended for individuals wishing an in-depth introduction to the F-techniques and their application to the life sciences. Participants are normally graduate students or Post-docs who are using fluorescence microscopy in their research. Most participants have some knowledge of fluorescence, typically in a specialized area. However, other individuals, from totally different research areas and industry, get the opportunity to enter this exciting field in a very effective way and benefit especially from the experimental section.
Course organisation
The course consists of lectures and practical sessions. Participation is possible by attending lectures only or attending lectures and practical sessions.
The lectures on Monday and Tuesday (July 16/17) will deal with fluorescence, theory of F-techniques (FRET, FLIM, FCS and FRAP), FCS variants, instrumentation and data analysis. The major FRET methods will be covered in detail. The lectures on Wednesday and Thursday (July 18/19) will cover research applications of F-techniques in cell and developmental biology. On Friday (July 20) lectures will focus on superresolution microscopy, PALM, STORM and PAINT.
Practical sessions (afternoons of July 17-20) will cover steady-state and time-resolved experiments as well as data analysis on various instruments provided by market leading companies.
A poster session/welcome reception will be held on the evening of the first day (Monday, July 16) to allow participants to meet instructors.
Course instructors (to be completed, subject to changes, in alphabetical order)
• Sohail Ahmed (IMB, Singapore)
• Andrew Clayton (Swinburne University, Australia)
• Andreas Herrmann (Humboldt University Berlin, Germany)
• Martin Hof (J. Heyrovsky Institute, Czech Republic)
• Sudipta Maiti (Tata Institute of Fundamental Research, India)
• Steffen Ruettinger (PicoQuant GmbH, Germany)
• Markus Sauer (Würzburg University, Germany)
• JB Sibarita (Bordeaux University, France)
• Sudha Thankiah (IMB, Singapore)
• Susanne Trautmann (PicoQuant GmbH, Germany)
• Malte Wachsmuth (EMBL, Germany)
• Thorsten Wohland (NUS, Singapore)
• Qing-Hua Xu (NUS, Singapore)
Practical sessions
The course schedule includes 4 practical sessions of 120 minutes each. The sessions cover different experimental F-techniques on different instruments. Participants will be divided into groups that rotate through the experimental stations.
Experimental techniques covered:
• FCS, FCCS and FLCS
• FLIM
• FRAP, AP-FRET
• FLIM-frequency domain
Please note that due to the expected number of participants, not every participant will have the possibility to work directly with the instruments. The practical sessions will be limited to a number of 24 participants, so early registration is recommended.
Location
The course will be hosted by the Institute of Medical Biology Microscopy Unit (IMU), based in Biopolis, Singapore. The course will be held at the Immunos/Neuros Complex, located at 8A Biomedical Grove, #06-06 Immunos, Singapore 138648.
For more information visit www.picoquant.com/_events.htm
Contact
PicoQuant GmbH
Rudower Chaussee 29 (IGZ)
12489 Berlin, Germany
Phone: +49-30-6392-6929
e-mail: events@picoquant.com
Institute of Medical Biology Microscopy Unit (IMU), Biopolis, Singapore
8A Biomedical Grove
Immunos, Singapore 138648
Phone:+65 6407 0202
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