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Leica Microsystems: Intravenous microinjection of zebrafish larvae to study acute kidney injury
  September 1, 2010 - Interesting Video about the zebrafish model for AKI from intravenous microinjection technique to visual measures of kidney disfunction
This video article describes a zebrafish model of acute kidney injury (AKI) upon intravenous microinjection of a nephrotoxicant. Microinjections have been performed under a Leica S6 E stereo microscope.
Successful microinjection was verified by monitoring lucifer yellow dextran fluorescence in the heart of the zebrafish using a Leica M205 FA stereo microscope.
The article provides a detailed protocol for intravenous microinjection and can be transferred to other types of studies that require delivering soluble substances into the bloodstream of zebrafish larvae.
Click here to see the video about the zebrafish model for AKI from intravenous microinjection technique to visual measures of kidney disfunction.
Leica S6 E
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Leica Microsystems is a leading global designer and producer of innovative, high-tech, precision optical systems for the analysis of microstructures. It is one of the market leaders in each of its business areas: Microscopy, Confocal Laser Scanning Microscopy with corresponding Imaging Systems, Specimen Preparation, and Medical Equipment. The company manufactures a broad range of products for numerous applications requiring microscopic imaging, measurement, and analysis. It also offers system solutions for life science including biotechnology and medicine, research and development of raw materials, and industrial quality assurance. The company is represented in over 100 countries with 14 manufacturing facilities in 11 countries, sales and service organizations in 19 countries and an international network of dealers. The international management is headquartered in Wetzlar, Germany.
www.leica-microsystems.com
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